DIG寡核苷酸5′末端标记试剂盒

来自 : 发布时间：2024-05-14

DIG寡核苷酸5′末端标记试剂盒 DIG Oligonucleotide 5′-End Labeling Set：Set for 10 labeling reactions 产品号 产品规格 厂商 价格 148086311480863001 Set (10个标记反应) Roche 5841 DIG Oligonucleotide 5-End Labeling SetApplicationThe set is used for 5-end labeling of oligonucleotides with digoxigenin. The oligonucleotide is reacted in a final synthesis step with an aminolinker phosphoramidite, creating a 5-terminal amino function. After completion of the oligonucleotide synthesis, the digoxigenin moiety is introduced at the 5 position.In general, 1 mg NHS-ester is sufficient for the labeling of approximately 300 nmol of amino-substituted oligonucleotide.Figure 1: Typical elution profile of a digoxigenin-labeled oligonucleotide. Product DescriptionStability: The compounds of the set are stable in solid form for at least one year when stored at 15 to 25°C. The phosphoramidite solution is stable for approximately two weeks at ambient temperature with the exclusion of moisture. The solution of the NHS-ester in ethanol is stable only for a short time. Therefore, the ester solution should be used for several parallel labelings.Contents1. \"Aminolinker” [N-Trifluoroacetamido-(3-oxa)-pentyl-N,N-diisopropyl-methyl]phosphoramidite, 100 mg 2.Digoxigenin-NHS-ester, (Digoxigenin-3-0-methylcarbonyl-e-aminocaproic acid-N-hydroxy-succinimide ester), 5 x 1 mg